Identification of Small Berry Species in Food and Juice Using TaqMan-Based Real-Time PCR
نویسندگان
چکیده
منابع مشابه
TaqMan real-time PCR: a reliable method to detect meat species
The aim of this study was to detect and identify meat species (beef, lamb, pork, chicken, donkey, and horse) in Kebab as a Halal meat-based food sold in on-road-restaurants of North Khorasan province (Iran) using TaqMan real-time polymerase chain reaction (PCR). Raw Kebab samples (150 samples) were obtained from on-road restaurants. The samples were prepared, DNA was extracted and TaqMan real-t...
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The polymerase chain reaction (PCR), first described in 1985, is a highly sensitive and specific technique used for the detection of nucleic acids [55]. The inventor of this technology earned a Nobel prize for his achievement [43,44], which has revolutionised research and diagnostic possibilities. Qualitative PCR is a well established and straightforward technology, but quantification of specif...
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background legionellaceae contains legionella genus with over 52 species and 64 serogroups. it is one of the most important causes of respiratory disease in human. more than 30% of hospital-acquired pneumonia is caused by legionella. ventilator-associated pneumonia (vap) is an infection acquired in hospital wards, particularly in intensive care unit (icu). this disease approximately affects 9% ...
متن کاملRapid identification of candida species by TaqMan PCR.
AIM To develop and evaluate a TaqMan(TM) polymerase chain reaction (PCR) for the rapid identification and speciation of candida species. METHODS Species specific primer and probe sets were designed for the identification of Candida albicans, C. parapsilosis, C. tropicalis, C. krusei, C. kefyr, and C. glabrata from clinical isolates in a 5' exonuclease (TaqMan(TM)) assay. The probes were label...
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ژورنال
عنوان ژورنال: Journal of AOAC INTERNATIONAL
سال: 2019
ISSN: 1060-3271,1944-7922
DOI: 10.1093/jaoac/102.5.1552